Chromosomal Aberrations in Irradiated Blood and Blood Cultures of Normal Subjects and of Selected Patients with Chromosomal Abnormality

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Abstract
Freshly drawn blood and also blood cultures were irradiated with a total dose of 100 rads. The blood was taken from 8 subjects, 3 of these were normal controls, and the remainder were patients with various chromosomal abnormalities. It was found that the rate of chromosome-type aberrations induced by irradiation of blood was significantly higher in the woman who was previously exposed to irradiation and whose control blood cultures had about 20% of "spontaneous" breaks. This was less striking in a mongoloid patient with fewer chromosomal aberrations in the control blood cultures. In the remainder of the mongoloids and in the patient with treated leukemia the rates of chromosome-type aberrations in the irradiated blood were not significantly different from those in the normal controls. The rates of chromatid-type aberrations induced by irradiation of blood cultures 4 hours prior to harvesting (G2) were not significantly different in the patients tested as compared to the normal controls. The relation of the number of acentric fragments to multicentric chromosomes in the first cell division was investigated.