Quantitative Determination of Cucurbitacins by High Performance Liquid Chromatography and High Performance Thin Layer Chromatography
- 1 January 1993
- journal article
- research article
- Published by Taylor & Francis in Journal of Liquid Chromatography
- Vol. 16 (2) , 497-511
- https://doi.org/10.1080/10826079308020927
Abstract
A simple and precise method using High-Performance liquid [HPLC] and High-Performance Thin-Layer Chromatography [HPTLC] was developed for simultaneous determination of the frequently encountered cucurbitacins B (1), D (2), 3-epi-iso-cucurbitacin D (3), E (4) and I (5) as well as cucurbitacin glycosides E (6) and I (7). A reverse phase column [C-18] was used and elution achieved with acetonitrile-water [2:8, solvent A and 45:55, solvent B] with gradient elution analysis. Reverse-phase HPTLC plates were developed with methanol: water (7:3) and normal-phase plates were developed with toluene-ethylacetate (25:75). Developed plates were scanned with Thin-Layer Scanning Instrumentation. A clean-up procedure was perfected before sample loading to both the HPLC and HPTLC.Keywords
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