Determination of Individual Carotenoids in Human Plasma by High Performance Liquid Chromatography
- 1 March 1985
- journal article
- research article
- Published by Taylor & Francis in Journal of Liquid Chromatography
- Vol. 8 (3) , 473-484
- https://doi.org/10.1080/01483918508067094
Abstract
A high performance liquid chromatography procedure using an internal carotenoid standard, echinenone, is described. The method uses a C-18, reverse-phase column, an isocratic solvent (acetonitrile:methylene chloride:methanol, 70:20:10) and requires only 13 min. Most human plasmas gave seven distinct peaks; 6 carotenoids are identified. The sum of 7 peaks is 90% or more of the total carotenoids in a total lipid extract.Keywords
This publication has 12 references indexed in Scilit:
- Simultaneous determination of plasma retinol, α-tocopherol, lycopene, α-carotene, and β-carotene by high-performance liquid chromatographyAnalytical Biochemistry, 1984
- Beta-carotene determined in serum by liquid chromatography with an internal standard.Clinical Chemistry, 1983
- Determination of lycopene, α‐ and β‐carotene and retinyl esters in human serum by reversed‐phase high performance liquid chromatographyLipids, 1983
- Isocratic nonaqueous reversed-phase liquid chromatography of carotenoidsAnalytical Chemistry, 1983
- High-performance liquid chromatography of the provitamin A β-carotene in plasmaJournal of Chromatography B: Biomedical Sciences and Applications, 1983
- DIETARY VITAMIN A AND RISK OF CANCER IN THE WESTERN ELECTRIC STUDYThe Lancet, 1981
- Can dietary beta-carotene materially reduce human cancer rates?Nature, 1981
- 814. Carotenoids and related compounds. Part VIII. Novel syntheses of echinenone and canthaxanthinJournal of the Chemical Society, 1959
- Isolation and nature of echinenone, a provitamin AArchives of Biochemistry and Biophysics, 1956