Laboratory apparatus and techniques for the rearing and insecticidal treatment of whiteflyBemisia tabaci(Homoptera: Aleyrodidae) under simulated field conditions

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Abstract
Laboratory apparatus and techniques are described for the rearing and insecticidal treatment of whitefly,Bemisia tabaciGennadius, under simulated field conditions. Insects were reared on cotton plants inside large population cages and treated from an overhead sprayer. The effects of these treatments were assessed accurately, without interfering with insects or plants, by monitoring adult numbers with an endoscope over one or more generations. Examples of single-generation and multiple-generation tests with cypermethrin are described. The apparatus is suitable for testing strategies for delaying the selection of resistance (e.g. using insecticides applied singly, alternately, or in mixture, at various application rates and frequencies), for controlling populations already resistant to insecticides, and for integrated pest management using chemical and biological control agents together.