The isolation and identification of 2,4-diketones present in human and animal tissues and in urine

Publisher Website
Google Scholar
Abstract
The chemical isolation of a 2,4-diketone fraction from hydrolysates of various mammalian tissues [beef brain, beef liver, human liver, human urine, human blood] was accomplished by the use of a modified Girard T procedure. This fraction, which constitutes a new lipid class [and which contains an antiallergic principle or principles], was resolved by gas chromatography into a number of homologous 2,4-diketones, ranging in chain length from C13-C25. After separation by preparative gas chromatography, the following compounds were unequivocally identified: 2,4-heptadecanedione, 2,4-nonadecanedione, 2,4-heneicosanedione, 2,4-docosanedione and .DELTA.12-2,4-heneicosenedione. The 2,4-diketones appear to exist in tissues in the free state or in labile combination. They are present also in human urine.