Extraction and High Performance Liquid Chromatographic Determination of Enrofloxacin in Fish Serum and Tissues

Abstract
The extraction of enrofloxacin from fish serum and tissues using solid phase extraction columns has been studied. Good recoveries were obtained by using C2 columns for cleanup of serum and C18 columns for clean-up of tissue samples. the sample pretreatment procedures are selective and robust having a sensitivity of 1 ng/ml and 1 ng/g from serum and tissues, respectively, making them particularly useful for monitoring drug residue levels. the recoveries were 97–100% from serum and 96–99% from tissues. the method was tested on fish treated with enrofloxacin.