Neuropeptide Y inhibits vasoactive intestinal peptide‐ and dopamine‐induced cyclic AMP formation in human Ewing's sarcoma WE‐68 cells
- 5 June 1989
- journal article
- Published by Wiley in FEBS Letters
- Vol. 249 (2) , 271-274
- https://doi.org/10.1016/0014-5793(89)80639-8
Abstract
Neuropeptide Y (NPY) regulation of intracellular cyclic AMP accumulation was studied in human Ewing's sarcoma cell line, WE-68. NPY inhibited vasoactive intestinal peptide (VIP)- and dopamine-stimulated but not basal cyclic AMP formation. The peptide effect was rapid (<2 min), concentration-dependent with a half-maximal effective concentration (EC50) of 8 nM NPY, and maximal inhibition reaching 60–70% with 100 nM NPY. Prior exposure of WE-68 cells to pertussis toxin completely abolished the inhibitory action of NPY. It is concluded that NPY attenuates agonist-stimulated cyclic AMP formation in Ewing's sarcoma WE-68 cells, and may do so via the inhibitory guanine nucleotide regulatory protein of adenylate cyclaseKeywords
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