Immunological crossreactivity to the catabolite control protein CcpA fromBacillus megateriumis found in many Gram-positive bacteria
Open Access
- 1 June 1996
- journal article
- Published by Oxford University Press (OUP) in FEMS Microbiology Letters
- Vol. 139 (2-3) , 109-115
- https://doi.org/10.1111/j.1574-6968.1996.tb08188.x
Abstract
The catabolite control protein CcpA from Bacillus megaterium was overproduced as a fusion protein to a 6xhis affinity tag and purified to homogeneity. Polyclonal antibodies of high affinity and specificity were raised against the purified protein. The serum did not crossreact with purified Lac repressor despite the fact that CcpA and LacI belong to the same protein family. Using this antiserum we identified proteins that share antigenic determinants with CcpA in many Gram-positive bacteria, including bacilli, staphylococci, streptococci, lactic acid bacteria, and some actinomycetes.Keywords
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