Major Outer Membrane Proteins of Escherichia coli Strains of Human Origin

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Abstract
The major outer membrane protein patterns of 45 E. coli strains of human origin were compared with that of E. coli K12 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Preparations of the former strains contained 2-5 major bands in the MW range between 30,000-42,000. The patterns were heterogeneous with respect to the numbers and electrophoretic mobilities of the major outer membrane protein bands. In all cases the fastest moving band was characterized as a protein similar to the ompA protein of strain K12 as it was partially degraded by trypsin and reacted specifically with antiserum against the purified ompA protein in a gel immunoradioassay. All other major membrane proteins are related to the ompC and ompF proteins (the porins) of strain K12 as they were peptidoglycan-associated and reacted with antisera against the purified ompC and/or ompF proteins.