Transfer of plasmid pE5-2 from Escherichia coli to Bacteroides gingivalis and B. intermedius
- 1 June 1989
- journal article
- research article
- Published by Wiley in Oral Microbiology and Immunology
- Vol. 4 (3) , 132-134
- https://doi.org/10.1111/j.1399-302x.1989.tb00239.x
Abstract
A unique shuttle plasmid, pE5-2, previously constructed to mediate gene transfer from Escherichia coli to colonic Bacteroides spp. has also been transferred via conjugation from E. coli to isolates of Bacteroides gingivalis and B. intermedius. The transfer occurred at a frequency of 1.4 to 2 .times. 10-7 per recipient. The presence of the plasmid in transconjugants was verified by hybridization of the total DNA of B. gingivalis recipients with sequences of the pE5-2 plasmid, as well as by standard plasmid isolation techniques.Keywords
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