12‐Hydroxyeicosatetraenoic acid upregulates P‐selectin‐induced tissue factor activity on monocytes

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Abstract
12‐Hydroxyeicosatetraenoic acid (12‐HETE), a product of the platelet lipoxygenase pathway, amplifies tissue factor expression by P‐selectin‐stimulated monocytes in a time‐ and dose‐dependent fashion. The same effect is observed when monocytes are incubated with Chinese hamster ovary cells transfected with the P‐selectin cDNA. Both 5‐HETE and leukotriene C4 are inactive in this system. Furthermore, the effect is not dependent on non‐specific monocyte adhesion, since monocytes incubated with CHO cells expressing E‐selectin do not express tissue factor, either in the presence or in the absence of 12‐HETE. These results show that 12‐HETE is a cofactor for the expression of tissue factor by monocytes.