In Vivo and Scanning Electron Microscopy Imaging of Upconverting Nanophosphors inCaenorhabditiselegans
Top Cited Papers
- 23 December 2005
- journal article
- letter
- Published by American Chemical Society (ACS) in Nano Letters
- Vol. 6 (2) , 169-174
- https://doi.org/10.1021/nl0519175
Abstract
We show here that upconversion phosphors can be imaged both by infrared excitation and in a scanning electron microscope. We have synthesized and characterized for this work up-converting phosphor nanoparticles nonaggregated nanocrystals of size range 50−200 nm. We have investigated the optical properties of 50−200 nm nanoparticles and found a square dependence of the emitted visible fluorescence on the infrared excitation and verified that under electron excitation similar narrow band emission spectra can be obtained as is seen with IR upconversion. The viability of the nanoparticles for biological imaging was confirmed by imaging the digestive system of the nematode worm Caenorhabditis elegans, and we have confirmed using energy-dispersive X-ray analysis that the up-conversion nanoparticles can be identified in a scanning electron microscope at high spatial resolution.Keywords
This publication has 7 references indexed in Scilit:
- Highly Efficient Multicolour Upconversion Emission in Transparent Colloids of Lanthanide‐Doped NaYF4 NanocrystalsAdvanced Materials, 2004
- Wet chemical synthesis and luminescence properties of erbium-doped nanocrystalline yttrium oxideJournal of Materials Research, 2004
- Upconversion and Anti-Stokes Processes with f and d Ions in SolidsChemical Reviews, 2003
- Up-converting phosphor reporters for nucleic acid microarraysNature Biotechnology, 2001
- Highly efficient infrared-to-visible energy upconversion in Er^3+:Y_2O_3Optics Letters, 2000
- C.elegansPublished by Oxford University Press (OUP) ,1999
- Cytotoxicity of the rare earth metals cerium, lanthanum, and neodymium in vitro: Comparisons with cadmium in a pulmonary macrophage primary culture systemEnvironmental Research, 1987