EFFECT OF SIMAZINE ON THE BIOLOGICAL ACTIVITY OF AZOTOBACTER CHROOCOCCUM

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Abstract
The effect of 10, 50, 100, 200, and 300 μg simazine per mL of culture medium or g of soil was studied in chemically-defined media, dialyzed-soil media, nonsterile soil incubated under aerobic conditions, and sterilized soil inoculated with Azotobacter chroococcum. The herbicide simazine did not affect microbial growth of A. chroococcum grown in either chemically-defined medium, dialyzed-soil medium or sterilized agricultural soil. However, the presence of 50 to 300 μg simazine per mL of culture or per g of soil had a stimulatory effect on dinitrogen fixation by Azotobacter in both nonsterile and sterile conditions. When the microorganism was cultured in the presence of simazine the cells contained higher levels of ATP compared with control cells. © Williams & Wilkins 1991. All Rights Reserved.

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