Identification of Proinsulin and C-Peptide in Human Serum by a Specific Immunoassay
- 1 September 1970
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 67 (1) , 148-155
- https://doi.org/10.1073/pnas.67.1.148
Abstract
An immunoassay for the measurement of human proinsulin and C-peptide is described. By a combination of this system and an insulin immunoassay, proinsulin, insulin, and C-peptide were identified in acid-ethanol extracts of serum after gel filtration. In keeping with the results of biosynthetic studies, insulin and C-peptide were found to be present in equimolar amounts. The absolute values of serum proinsulin determined independently in the two assays corresponded closely.Keywords
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