Structural organization and expression of human DNA sequences related to the transforming gene of avian myeloblastosis virus.

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Abstract
Bacteriophage libraries of human DNA were screened for sequences homologous to the transforming gene (v-myb) of avian myeloblastosis virus. The 3 overlapping clones isolated were shown to contain a total of 1.0 kilobase pair (kbp) of sequence related to v-myb distributed over 6.2 kbp. Restriction enzyme mapping and heteroduplex analysis revealed the presence of 5 myb-related domains interrupted by 4 stretches of nonhomology. To study the extent of human DNA coding sequences that constitute the myb gene homologue, c-myb (human), probes spanning .apprx. 30 kbp were prepared from the clones and used to study transcription in a human hematopoietic cell line (MOLT-4). Each of the probes hybridized a 4.5-kilobase transcript, which suggests that either the c-myb (human) gene encompasses 30 kbp or it contains .gtoreq. 2 transcription units that each give rise to a mRNA of 4.5 kilobases.