Moloney murine sarcoma virions synthesize full-genome-length double-stranded DNA in vitro.

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Abstract
Moloney murine sarcoma virus (MSV) virions incubated under optimal conditions support extensive synthesis of double-stranded DNA. The major product, a 5950-base-pair (6-kilobase-pair DNA) double-stranded DNA, was characterized by cleavage with [Hae II, HindIII] restriction endonucleases and contained a 600-nucleotide-long direct repeat at both ends of the MSV genome. Linear DNA molecules made in vivo shortly after infection were compared to the linear double-stranded DNA synthesized in vitro. The restriction maps of both viral DNA products were indistinguishable. The 600-base-pair repeat results in a progeny DNA molecule that is longer than the parental MSV genomic RNA. The generation of this repeat must involve a mechanism that allows the viral reverse transcriptase (RNA-dependent DNA nucleotidyltransferase) to copy 5''- and 3''-terminal genomic (+) strand sequences twice.