Lectin grouping of Bacillus thuringiensis serovars

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Abstract
Twelve lectins, 11 of plant and 1 of animal origin, were tested against the 28 serovars of Bacillus thuringiensis to study their agglutinating specificities. Except for the sialic specific lectin from Limulus polyphemus, tube agglutination assays were performed using lectin concentrations of 10, 50, and 100 μg against 0.2 mL of 109 cells/mL in a final volume of 1.0 mL. The agglutination studies with the Limulus lectin were performed using 10 and 50 μg of lectin. Tubes were incubated overnight at room temperature (25 °C) and observed for aggutination patterns. Ten of the 28 serovars were individualized according to their ability to bind with various lectins. The study shows that the various serovars have different carbohydrate residues which indicates that the O-somatic antigens differ.

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