Stabilization of clathrin coated vesicles by amantadine, tromantadine and other hydrophobic amines
- 9 April 1991
- journal article
- Published by Wiley in FEBS Letters
- Vol. 281 (1-2) , 188-190
- https://doi.org/10.1016/0014-5793(91)80390-o
Abstract
Amantadine and related compounds stabilized the structure of purified pig brain clathrin coated vesicles (CCV) at biologically relevant concentrations. Incubation of purified CCV for 30 min at 25°C or 37°C caused the release clathrin, as determined by a centrifugation assay, and a reduction in the number of coated vesicles, by electron microscopy. Amantadine (10 mM), tromantadine (1 mM), amidine D295 (cyclohexylcarboximidamide‐(N‐benzyl)hydrochloride) (10 mM), chloroquin (0.1 mM) and monodansyleadaverine (10 mM) significantly reduced the extent of dissociation.Keywords
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