SIMULTANEOUS DETERMINATION OF THE PLASMA VOLUME WITH T-1824, AND THE "AVAILABLE FLUID" VOLUME WITH SODIUM THIOCYANATE

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Abstract
Directions for carrying out simultaneous detns. of plasma vol. with the blue dye, T-1824, and of "available fluid" with thiocyanate are briefly outlined. The results of preliminary tests show that these methods may be combined without affecting the accuracy of either the T-1824 or the thiocyanate analysis. By determining the thiocyanate (as ferric thiocyanate) as well as the dye conc. with a spectrophotometer, both analyses may be completed on 0.5 cc. of serum. Spectrophotometric technic, further-more, eliminates the errors encountered with colorimeters when the plasma vol. and "available fluid" determinations are repeated and the blood contains "residual" dye or thiocyanate. In the dog there are large individual differences in both plasma vol. and "available fluid" per kgm. body wt. Under normal conditions the plasma vol. is a function of the total vol. of "available fluid." As the animals mature, they show a striking decrease in the vol. of plasma and "available fluid" per kgm. body wt. [female][female] appear to have more "available fluid" relative to plasma than [male][male].

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