Isolation and characterization of 15‐hydroxylated metabolites of leukotriene C4

Abstract
A polar metabolite of leukotriene C4 was formed by sequential conversions with soybean lipoxygenase I and liver peroxidase. The structure of this product was found to be 5(S), 15(S)‐dihydroxy‐6(R)‐S‐glutathionyl‐7,9,13‐trans‐11‐cis‐eicosatetraenoic acid (15‐hydroxy‐Δ13trans‐leukotriene C3). The HPLC behaviour, the molar extinction coefficient and the biological activity of the metabolite are reported. Preliminary evidence suggests that this product is formed by mammalian tissues.

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