Observations on the results of specific histochemical techniques and empirical staining methods on several tissue/organ types using a variety of fixing fluids
- 1 July 1979
- journal article
- research article
- Published by Wiley in Histopathology
- Vol. 3 (4) , 321-327
- https://doi.org/10.1111/j.1365-2559.1979.tb03013.x
Abstract
Several histochemical techniques (Harris hematoxylin and alcoholic eosin, Gomori''s reticulum method, Verhoeff''s elastic stain; Gomori''s trichrome, Mallory''s aniline blue collagen stain and the Cameron and Steele method) were used with fixatives (Lavdowsky''s chromic acid, Karnovsky''s glutaraldehyde/paraformaldehyde, neutral buffered formalin, Lavdowsky''s formalin-acetic acid-alcohol, Zenker''s and Helley''s). Results with Lavdowsky''s chromic acid fixative were superior to the other fixing fluids and it was more sensitive than trichrome or connective tissue staining procedures. The Cameron and Steele modification used with Lavdowsky''s chromic acid fixative gave the best differentiation of tissue types and was the most reproducible. [Tissues examined included heart, lung, kidney, liver, pancreas, spleen, intestine and skeletal muscle from C57 BL/6, C3 Hf and Swiss albino mice].Keywords
This publication has 2 references indexed in Scilit:
- A rapid trichrome staining procedure for the identification of tissue typesJournal of Molecular Histology, 1979
- Simplified Aldehyde-Fuchsin Staining of Neurosecretory CellsStain Technology, 1959