LOW TEMPERATURE PRESERVATION OF BOAR SPERMATOZOA

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Abstract
Three freezing trials were conducted on the sperm-rich fraction of extended boar semen to examine the effects of various freezing methods, cryoprotective agents, and packaging containers on recovery of motile spermatozoa.Four freezing methods were employed: pellet freeze, nitrogen vapor freeze, fast mechanical alcohol freeze and slow mechanical alcohol freeze. Of these, the slow mechanical alcohol freeze resulted in highest recovery rates. In all three trials, the slower freezing rate resulted in a higher recovery of spermatozoa on thawing.Glycerol and NN-dimethylacetamide were employed, individually and in combination, as cryoprotective agents. The use of glycerol resulted in significantly higher recovery than NN-dimethylacetamide, but a combination of the two appeared to give good results.A comparison of plastic straws and glass ampoules as containers for frozen semen demonstrated no significant differences with respect to spermatozoan recovery.No fertility was obtained following the insemination of 14 animals with frozen semen containing a combination of glycerol and NN-dimethylacetamide as cryoprotective agents.

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