Identification of bovine T and B lymphocytes in normal peripheral blood, lymph nodes and spleen.

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Abstract
Anti-bovine thymocyte serum (ATS) was prepared by immunizing rabbits with calf thymocyte, adsorbed by normal bovine red blood cells, liver powder and bone marrow cells, and applied to identify bovine T lymphocytes by indirect immunofluorescent antibody technique (FA). The frequency of T and B lymphocytes in the PBL [peripheral blood lymphocyte], spleen and lymph nodes of 21 clinically normal cattle were investigated by indirect FA using ATS and by direct FA using FITC [fluorescein isothiocyanate] rabbit anti-bovine serum, respectively. Distributions of T and B lymphocytes were 66.4 .+-. 6.4% and 25.4 .+-. 3.9% in the PBL, 43.4 .+-. 4.8% and 48.7 .+-. 4.7% in the spleen and 59.4 .+-. 6.8% and 28.2 .+-. 5.6%, respectively, in the lymph nodes.

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