Fluorescence immunohistochemical detection of hypoxic cells in spheroids and tumours
Open Access
- 1 December 1987
- journal article
- research article
- Published by Springer Nature in British Journal of Cancer
- Vol. 56 (4) , 395-400
- https://doi.org/10.1038/bjc.1987.213
Abstract
Polyclonal antibodies have been raised in rabbits to a haemocyanin adduct of a reductively-activated, fluorinated analogue of misonidazole. Fluorescence immunohistochemical studies show that the polyclonal antibodies bind to spheroid sections and tumour sections in patterns similar to those revealed by autoradiographic studies with a tritium-labelled derivative of the fluorinated misonidazole analogue.Keywords
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