The choice of the suspension medium for rat-liver-cell nuclei

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Abstract
For isolation of rat-liver nuclei a medium is recommended having this composition: 0.3 [image] sucrose, 0.0652 [image] potassium glycerophosphate, 0.001 [image] MgCl2, 40% glycerol; pH 7.0. Phase-contrast photographs are shown of nuclei in this medium, and in modifications of it causing various abnormal appearances. Nuclei in the recommended medium retain their normal appearance for at least 6 hours at room temperature, after which they are still able to form haloes of soluble viscous nucleic acid when transferred to molar NaCl. If the glycerophosphate concentration is increased to 0.115 [image], with or without MgCl2, nuclei retain their normal appearance for 100 hours at room temperature, apart from slight clumping; but the higher ionic strength of this medium may sometimes be a disadvantage.
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