Specific Effects of Synthetic Oligopeptides on Cultured Animal Cells
- 1 January 2002
- journal article
- Published by Wiley in Biotechnology Progress
- Vol. 18 (1) , 155-158
- https://doi.org/10.1021/bp0101278
Abstract
Synthetic oligopeptides, tri- to pentaglycine and tri- and tetraalanine, were found to enhance viable cell density and culture viability when applied at concentrations higher than milllimolar to the cultures of a model hybridoma line. Oligoalanines, in addition, enhanced monoclonal antibody yields. Oligoglycines promoted solely the cell growth, unless the batch culture was fed with a medium concentrate. Examination of the effects of various tripeptides composed of glycine, alanine, serine, threonine, lysine, and histidine showed that some of the peptides promoted the growth of the culture, while other peptides suppressed the growth and enhanced the monoclonal antibody yield. Determination of the levels of amino acids and peptides in culture media indicated that the observed changes of culture parameters were caused by intact peptide molecules, rather than by amino acids liberated from the peptides by enzymic cleavage.Keywords
This publication has 11 references indexed in Scilit:
- Plant Protein Hydrolysates: Preparation of Defined Peptide Fractions Promoting Growth and Production in Animal Cells CulturesBiotechnology Progress, 2000
- The use of peptones as medium additives for the production of a recombinant therapeutic protein in high density perfusion cultures of mammalian cellsCytotechnology, 2000
- Protection of B lymphocyte hybridoma against starvation-induced apoptosis: survival-signal role of some amino acidsImmunology Letters, 1996
- The protein hydrolysate, Primatone RL, is a cost-effective multiple growth promoter of mammalian cell culture in serum-containing and serum-free media and displays anti-apoptosis propertiesJournal of Immunological Methods, 1996
- Cell suicide in starving hybridoma culture: survival-signal effect of some amino acidsCytotechnology, 1996
- Peptone, a low-cost growth-promoting nutrient for intensive animal cell cultureCytotechnology, 1994
- Cell retention–chemostat studies of hybridoma cells—analysis of hybridoma growth and metabolism in continuous suspension culture in serum‐free mediumBiotechnology & Bioengineering, 1993
- Fragmented DNA and apoptotic bodies document the programmed way of cell death in hybridoma culturesCytotechnology, 1992
- On-line monitoring of monoclonal antibody formation in high density perfusion culture using FIACytotechnology, 1991
- The growth-stimulating activity of peptides on human hematopoietic cell culturesExperimental Cell Research, 1969