NONFUNCTIONAL NATURE OF SULFHYDRYL GROUPS FOR PIGEON LIVER MALIC ENZYME
- 1 October 1980
- journal article
- research article
- Published by Wiley in International Journal of Peptide and Protein Research
- Vol. 16 (4) , 321-326
- https://doi.org/10.1111/j.1399-3011.1980.tb02593.x
Abstract
The cyanylated derivative of pigeon liver malic enzyme (EC 1.1.1.40) may be prepared by reacting the SH [sulfhydryl] groups of the enzyme with 2-nitro-5-thiocyanobenzoic acid in the presence of excess KCN or by replacing the thionitrobenzoate moiety of the thionitrobenzoated enzyme with CN group. The -SCN derivative is enzymatically active and is resistant to react with the affinity label bromopyruvate. In the generation of -SCN enzyme from the thionitrobenzoated enzyme, there is a correlation between the [14C] CN incorporation and the activity recovered. The SH groups are not directly involved in the catalytic mechanism of the malic enzyme. Evidence is provided to show that the SH groups were not involved in the binding of nucleotide coenzyme.Keywords
This publication has 13 references indexed in Scilit:
- Involvement of tyrosyl residues in the substrate binding of pigeon liver malic enzymeBiochimica et Biophysica Acta (BBA) - Enzymology, 1980
- Inactivation of bovine pancreatic DNase by 2-nitro-5-thiocyanobenzoic acid. I. A novel inhibitor for DNase I.Journal of Biological Chemistry, 1979
- Inactivation of DNase by 2-nitro-5-thiocyanobenzoic acid. II. Serine and threonine are the sites of reaction on the DNase molecule.Journal of Biological Chemistry, 1979
- Oxidized NADP as a potential active-site-directed reagent of pigeon liver malic enzymeBiochemical and Biophysical Research Communications, 1979
- Mechanism of pigeon liver malic enzyme. Reactivity of class II sulfhydryl groups as conformational probe for the "half-of-the-sites" reactivity of the enzyme with bromopyruvateBiochemistry, 1978
- Mechanism of pigeon liver malic enzyme modification of histidyl residues by ethoxyformic anhydrideBiochimica et Biophysica Acta (BBA) - Enzymology, 1977
- Mechanism of pigeon liver malic enzyme. Kinetics, specificity, and half-site stoichiometry of the alkylation of a cysteinyl residue by the substrate-inhibitor bromopyruvateBiochemistry, 1977
- Alternative products in the reaction of 2-nitro-5-thiocyanatobenzoic acid with thiol groupsBiochemical Journal, 1976
- Mechanism of Pigeon Liver Malic Enzyme. Modification of Sulfhydryl Groups by 5,5′-Dithiobis(2-nitrobenzoic Acid) and N-EthylmaleimideJournal of Biological Chemistry, 1974
- Mechanism of pigeon liver malic enzyme subunit structureBiochimica et Biophysica Acta (BBA) - Protein Structure, 1974