The Class II Transactivator Requires brahma-Related Gene 1 To Activate Transcription of Major Histocompatibility Complex Class II Genes
Open Access
- 1 July 2002
- journal article
- Published by Taylor & Francis in Molecular and Cellular Biology
- Vol. 22 (14) , 5019-5026
- https://doi.org/10.1128/mcb.22.14.5019-5026.2002
Abstract
The class II transactivator (CIITA) is the key regulator of major histocompatibility complex (MHC) class II gene transcription. We demonstrate here that CIITA requires the ATPase subunit of an hSWI/SNF complex, brahma-related gene 1 (BRG-1), to activate transcription. When introduced into a cell line lacking BRG-1, CIITA was unable to activate cellular MHC class II genes. Reexpression of the wild-type but not an ATP-binding-deficient BRG-1 protein in this cell line restored the ability of CIITA to transactivate transcription of MHC class II genes. Interestingly, when the activity of CIITA was assayed in the BRG-1-deficient cell line by using a plasmid-based reporter assay, BRG-1 was not required for transcriptional activation, suggesting that the chromatin structure on the plasmid is such that BRG-1 is not necessary. Coimmunoprecipitation experiments were performed to determine if BRG-1 and CIITA proteins associate with each other in cells. We found that the two proteins coimmunoprecipitate and that amino acids 1 to 140 of CIITA are sufficient for binding. Taken together, these data suggest that BRG-1 and, very likely, an hSWI/SNF complex are required for transcription of MHC class II genes. The complex is likely recruited to MHC class II promoters, at least in part, by interaction with CIITA.Keywords
This publication has 62 references indexed in Scilit:
- Histone Deacetylase Activity Represses Gamma Interferon-Inducible HLA-DR Gene Expression following the Establishment of a DNase I-Hypersensitive Chromatin ConformationMolecular and Cellular Biology, 2001
- The Histone Acetyltransferase Domains of CREB-binding Protein (CBP) and p300/CBP-associated Factor Are Not Necessary for Cooperativity with the Class II TransactivatorPublished by Elsevier ,2001
- Site-Specific Acetylation by p300 or CREB Binding Protein Regulates Erythroid Krüppel-Like Factor Transcriptional Activity via Its Interaction with the SWI-SNF ComplexMolecular and Cellular Biology, 2001
- Reciprocal Modulation of Transcriptional Activities between HIV-1 Tat and MHC Class II Transactivator CIITABiochemical and Biophysical Research Communications, 2000
- HLA class II expression in human tumor linesMicrobes and Infection, 1999
- pRB is required for interferon-γ-induction of the MHC class II Aβ geneOncogene, 1999
- IFN‐γ inducibility of class II transactivator is specifically lacking in human tumour lines: Relevance to retinoblastoma protein rescue of IFN‐γ inducibility of the HLA class II genesImmunology & Cell Biology, 1997
- RB and A novel E2F‐1 binding protein in MHC class II deficient B‐cell lines and normal IFN‐γ induction of the class II transactivator ciita in class II non‐inducible RB‐defective tumor linesInternational Journal of Cancer, 1995
- Nucleosome disruption and enhancement of activator binding by a human SW1/SNF complexNature, 1994
- Sequences and Factors: A Guide to MHC Class-II TranscriptionAnnual Review of Immunology, 1992