Flow injection determination of thiamine based on its oxidation to thiochrome by mercury(II)
- 1 January 1990
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in The Analyst
- Vol. 115 (2) , 217-220
- https://doi.org/10.1039/an9901500217
Abstract
The reaction involving the oxidation of thiamine to fluorescent thiochrome by mercury(II) in basic solutions has been adapted to flow injection. Linear calibration graphs were obtained between 2 × 10–7 and 7 × 10–6 M, with a sampling rate of 22 samples h–1 and a relative standard deviation of 0.42–0.17%. The applicability of the method to the determination of thiamine in vitamin-mineral preparations was demonstrated by investigating the effect of potential interferences and by the analysis of commercial preparations.Keywords
This publication has 13 references indexed in Scilit:
- Fluorometric reaction rate method for the determination of thiamineAnalytical Chemistry, 1980
- Extraction based on the flow-injection principle: Part 3. Fluorimetric Determination of Vitamin B1 (Thiamine) by the Thiochrome MethodAnalytica Chimica Acta, 1980
- An improved procedure for the determination of thiamineThe Analyst, 1975
- Simultaneous Determination of Thiamine and 2-(1-Hydroxyethyl) thiamine in Biological MaterialsTHE JOURNAL OF VITAMINOLOGY, 1969
- The effect of methyl alcohol on the conversion of thiamine to thiochromeCellular and Molecular Life Sciences, 1960
- Determination of Thiamine by Thiochrome ReactionAnalytical Chemistry, 1953
- An extrapolation procedure for the assay of aneurineThe Analyst, 1952
- 113. Aneurin. Part X. The mechanism of thiochrome formation from aneurin and aneurin disulphideJournal of the Chemical Society, 1951
- A new method for the oxidation of aneurin to thiochrome and a procedure for the determination of aneurin in oatsBiochemical Journal, 1944
- Quenching effect of electrolytes on the fluorescence intensity of riboflavin and thiochromeBiochemical Journal, 1944