Linkage of loci associated with two pigment mutations on mouse chromosome 13

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Abstract
Summary: Progeny from one intra- and two inter-specific backcrosses between divergent strains of mice were typed to map multiple markers in relation to two pigment mutations on mouse chromosome 13, beige (bg) and pearl (pe). Both recessive mutants on a C57BL/6J background were crossed separately with laboratory strain PAC (M. domesticus) and the partially inbredM. musculusstock PWK. The intra- and inter-specific F1hybrids were backcrossed to the C57BL/6J parental strain and DNA was prepared from progeny. Restriction fragment length polymorphisms were used to follow the segregation of alleles in the backcross offspring at loci identified with molecular probes. The linkage analysis defines the association between thebgandpeloci and the loci for the T-cell receptor γ-chain gene (Tcrg), the spermatocyte specific histone gene (Hist 1), the prolactin gene (Prl), the Friend murine leukaemia virus integration site 1 (Fim-1), the murine Hanukuh Factor gene (Muhf/Ctla-3) and the dihydrofolate reductase gene (Dhfr). This data confirms results of prior chromosomal mapping studies utilizingbgas an anchor locus, and provides previously unreported information defining the localization of the prolactin gene on mouse chromosome 13. The relationship of multiple loci in relation topeis similarly defined. These results may help facilitate localization of the genes responsible for two human syndromes homologous withbgandpe, Chediak–Higashi syndrome and Hermansky–Pudlak syndrome.