A rapid method for the assay of dextranase
- 1 March 1970
- journal article
- Published by Canadian Science Publishing in Canadian Journal of Biochemistry
- Vol. 48 (3) , 225-227
- https://doi.org/10.1139/o70-041
Abstract
A method, based on the liberation of a dye complex from Blue Dextran 2000, is described for the assay of dextranase. The reaction was conducted at 40 °C for 5 min at pH 4.0 in a mixture containing 0.5% Blue Dextran 2000 and the enzyme. The Km for the hydrolysis of Blue Dextran 2000 was estimated at 2.5 × 10−6 M.Keywords
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