A precursor of cytoplasmic actin in cultured Drosophila cells.
Open Access
- 1 June 1979
- journal article
- research article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 81 (3) , 680-683
- https://doi.org/10.1083/jcb.81.3.680
Abstract
In continuous lines of cultured Drosophila cells 2 forms of cytoplasmic actin, designated II and III, are detected after a 25-120 min pulse label with [35S]methionine. Only actin II accumulates in the cell. With the use of a pulse-chase protocol and 2 dimensional gel electrophoresis, it was found that actin III is synthesized as a precursor of the more stable cytoplasmic actin II. The half-life of actin III was estimated at approximately 50 min.Keywords
This publication has 4 references indexed in Scilit:
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- Characterization of the mRNAs for α-, β- and γ-actinCell, 1977
- Identification and characterization of multiple forms of actinCell, 1976