A preparatory technique for examination of imperfect fungi by scanning electron microscopy.

1 January 1975

journal article

Vol. 12 (46) , 115-21

Abstract

Thin 12 mm diameter coverslips, coated with nutrient agar, were inoculated with a fungus, incubated, and sequentially examined with the light microscope and then the scanning electron microscope (SEM), thus providing the valuable capability of correlation of results obtained from these two microscopic analyses. A sandwich of two coverslips was prepared for light-microscopic observations, and then separated and the agar-coated coverslip on which the fungus had grown was passed through fixative solutions, critical point dried, metal-coated and examined in the SEM. The technique was designed primarily for studies of conidiogenesis in rapidly growing human pathogenic imperfect fungi.

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