Carboxyxmethylcellulose Chromatography in 6M Urea and Zone Electrophoresis of Calf Thymus Histone*

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Abstract
Calf thymus histone was chromatographed on a carboxymethylcellulose column eluted with a gradient of salt concentration in buffer containing 6 M urea.Whole histone was eluted in more than 4 peaks with up to 0.15 M potassium chloride in 0.04 M sodium acetate-6 M urea, pH 5.5. The order of elution of the histone fractions differed from those reported previously. Four histone fractions obtained by the method of Johns, arginine-rich, intermediate, slightly lysine-rich and very lysinerich, were further resolved with the present chromatographic system.Further fractionation of the lysine-rich histone fraction was achieved by zone electrophoresis on a vertical column. A very lysine-rich histone, which was homogeneous electrophoretically, was obtained on a preparative scale.

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