Simultaneous Spectrophotometric Determination of Sulfamethoxazole and Trimethoprim in Drugs.

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Abstract
Two methods, namely first derivative and classical least squares methods are selected and applied for comparative purposes to analyze uv-spectra of the methanolic solutions of the cited components in synthetic binary mixtures and in a number of antibacterial pharmaceutical preparations, viz. three tablets and two syrups produced by Egyptian companies. The former technique is based on measuring the absorbances at zero-crossing wavelengths, 288 and 240 nm for the two drugs, respectively. Calibration curves are rectilinear in the range 4 –20 μg/ml of both components. The latter method is based on a pure standard full-spectra treatment ranged from 350 to 200 nm at 2 nm intervals to compute the concentration of unknowns. A statistical analysis of the results is reported.