High Performance Liquid Chromatography of Protein. V. Adsorption of Insulin on Glass Surfaces

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Abstract
Conventionally, insulin was determined by using radioimmunoassay and radioisotope methods. We established the determination of insulin by high performance liquid chromatography, which is superior to another assay methods on simplicity, rapidity and accuracy, and identification limit of insulin is 0.5 ng. By using this method, we investigated the adsorption of insulin on glass surfaces. Adsorption of insulin was little independent upon pH and ionic strength. It was suggested that adsorption of insulin to glass surfaces was mainly hydrophobic interaction.

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