The Human Immunodeficiency Virus Transactivator Tat Interacts with the RNA Polymerase II Holoenzyme
Open Access
- 1 April 1997
- journal article
- Published by Taylor & Francis in Molecular and Cellular Biology
- Vol. 17 (4) , 1817-1823
- https://doi.org/10.1128/mcb.17.4.1817
Abstract
The human immunodeficiency virus (HIV) encodes a transcriptional transactivator (Tat), which binds to an RNA hairpin called the transactivation response element (TAR) that is located downstream of the site of initiation of viral transcription. Tat stimulates the production of full-length viral transcripts by RNA polymerase II (pol II). In this study, we demonstrate that Tat coimmunoprecipitates with the pol II holoenzyme in cells and that it binds to the purified holoenzyme in vitro. Furthermore, Tat affinity chromatography purifies a holoenzyme from HeLa nuclear extracts which, upon addition of TBP and TFIIB, supports Tat transactivation in vitro, indicating that it contains all the cellular proteins required for the function of Tat. By demonstrating that Tat interacts with the holoenzyme in the absence of TAR, our data suggest a single-step assembly of Tat and the transcription complex on the long terminal repeat of HIV.Keywords
This publication has 40 references indexed in Scilit:
- A human RNA polymerase II complex associated with SRB and DNA-repair proteinsNature, 1996
- A mammalian RNA polymerase II holoenzyme containing all components required for promoter-specific transcription initiationCell, 1995
- HIV-1 nef leads to inhibition or activation of T cells depending on its intracellular localizationImmunity, 1994
- CONTROL OF RNA INITIATION AND ELONGATION AT THE HIV-1 PROMOTERAnnual Review of Biochemistry, 1994
- Dual role of TFIIH in DNA excision repair and in transcription by RNA polymerase IINature, 1994
- Direct interaction of human TFIID with the HIV-1 transactivator TatNature, 1994
- HIV-1 Tat acts as a processivity factor in vitro in conjunction with cellular elongation factors.Genes & Development, 1992
- Two distinct nuclear transcription factors recognize loop and bulge residues of the HIV-1 TAR RNA hairpin.Genes & Development, 1991
- The HIV-1 Tat protein activates transcription from an upstream DNA-binding site: implications for Tat function.Genes & Development, 1991
- Human Chromosome 12 Is Required for Elevated HIV-1 Expression in Human-Hamster Hybrid CellsScience, 1989