THE EFFECT OF A PLASMA-PROTEIN FRACTION ON LIPID-SYNTHESIS IN CULTURED SKIN FIBROBLASTS FROM NORMALS AND PATIENTS WITH HYPERAPOBETALIPOPROTEINEMIA

  • 1 April 1988
    • journal article
    • research article
    • Vol. 11  (2) , 99-107
Abstract
Lipid synthesis was measured in cultured skin fibroblasts obtained from normals and patients with Hyperapobetalipoproteinemia (HyperapoB). Using lipoprotein-deficient serum medium, triglyceride synthesis and cholesterol esterification were greater in normals than in HyperapoB due to differences in de novo synthesis, not to differences in re-esterification or to different rates of hydrolysis. When normal and HyperapoB cells were incubated in serum-free medium, however, lipid synthesis was the same. Serum was then fractionated chromatographically and a partially purified protein fraction shown to be responsible for the stimulatory effect in normals.

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