Engineering of microheterogeneity‐resistant p‐hydroxybenzoate hydroxylase from Pseudomonas fluorescens
- 17 December 1990
- journal article
- Published by Wiley in FEBS Letters
- Vol. 277 (1-2) , 197-199
- https://doi.org/10.1016/0014-5793(90)80843-8
Abstract
By site‐directed mutagenesis, Cys‐116 was converted to Ser‐116 in p‐hydroxybenzoate hydroxylase (EC 1.14.13.2) from Pseudomonas fluorescens. In contrast to wild‐type enzyme, the C116S mutant is no longer susceptible to oxidation by hydrogen peroxide and shows no reactivity towards 5,5'‐dithiobis(2‐nitrobenzoate). Crystals of the C116S mutant are isomorphous with the crystal form of wild‐type enzyme. A difference electron density confirms the mutation made.Keywords
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