Abstract
A simple, modified formulation for urea broth gave consistent, reliable results to aid in the differentiation of Mycobacterium spp. In a study of 1346 isolates representing 17 different species, tests read after 7 days were distinct and reproducible. The use of this test facilitates the identification of Mycobacterium spp., such as M. scrofulaceum, the M. avium complex, the M. terrae complex and M. triviale.