Relative DNA Damage Induced in Cultured Human Skin Fibroblasts by Exposure to the Precarcinogen 2-Acetylaminofluorene, the Proximate Carcinogen N-Hydroxy-2-acetylaminofluorene, and the Ultimate Carcinogen N-Acetoxy-2-acetylaminofluorene

1 July 1973

journal article
Published by Canadian Science Publishing in Canadian Journal of Biochemistry

Vol. 51 (7) , 990-994
https://doi.org/10.1139/o73-128

Abstract

DNA damage in cultured human skin fibroblasts was induced by the ultimate carcinogen N-acetoxy-2-acetylaminofluorene at concentrations as low as 2 × 10⁻⁶ M. The precarcinogen 2-acetylaminofluorene and the proximate carcinogen N-hydroxy-2-acetylaminofluorene failed to induce measurable DNA damage. The extent of DNA damage was measured by a technique of velocity sedimentation through alkaline sucrose gradients; it increased with the applied concentration of N-acetoxy-2-acetylaminofluorene and the carcinogen 4-nitroquinoline-1-oxide. Measurement of DNA damage induced by 30-min exposure to 4 × 10⁻⁷ M 4-nitroquinoline-1-oxide indicated that the technique employed in this study is of greater sensitivity than those previously reported.

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