Inhibitory influence of amino acids on Secretagogues induced exocrine secretion in isolated perfused rat pancreas.
- 31 December 1983
- journal article
- research article
- Published by Physiological Society of Japan in The Japanese Journal of Physiology
- Vol. 34 (1) , 167-180
- https://doi.org/10.2170/jjphysiol.34.167
Abstract
Influence of amino acids upon pancreatic exocrine secretion was investigated in the isolated perfused pancreas of rats. Arg produced significant and dose-related inhibition of pancreatic juice flow, protein output and amylase output evoked by CCK-PZ [cholecystokinin-pancreozymin] (1.25 pM). The secretory response evoked by CCK-PZ was inhibited by other amino acids (Ala, Asp, Asn, Gly, Iel, Leu, Lys, Met, Phe, Pro, Thr, Trp, Val, in each 20 mM). A similar inhibitory pattern was observed using 10 mixed amino acids of 2 mM each (Pro, Phe, Thr, Met, Lys, Asp, Leu, Trp, Val, Gly). Gly at a concentration of 20 mM produced significant inhibition of exocrine secretion evoked by acetylcholine (50 nM) or gastrin releasing peptide (36 pM). The inhibitory response induced by amino acids could not be repeated by using exogenous insulin (1 .mu.M) and glucagon (280 nM). The inhibitory response was also not changed by increased extracellular Ca (5 or 10 mM). However, Gly (20 mM) produced inhibition of exocrine secretion evoked by Ca reintroduction into a pancreas which was pretreated with A23187 [calcimycin]. The inhibition effects of some amino acids on exocrine secretion are apparently mainly caused by suppression of Ca influx in a stimulus-secretion coupling process.Keywords
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