Effect of Horse Serum and Fetal Calf Serum on Stability of Chromosomes in Mouse Embryo Cells In Vitro2

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Abstract
Horse serum and fetal calf serum as supplements to chemically defined medium differentially influenced the stability of chromosomes in mouse embryo cells during growth in vitro. Cells from both C3Hf/HeN and germfree ALB-2T mice showed more chromosomal abnormalities in horse serum-supplemented medium than in Fetal calf-serum medium. Studies were carried out to explain the mechanism controlling this difference in karyotypic stability in the two types of serum. Four possibilities were discussed. Experiments on transfer of cells after 458 days in horse-serum medium to Fetal calf-serum medium indicated that fetal calf serum did not act selectively to eliminate cells with abnormal karyotype. Further, fetal calf serum appeared not to select from the initial mixed population cells less subject to chromosomal alteration. Instead, horse serum seemed to induce chromosomal breaks at a faster rate than did fetal calf serum. Also, it appeared that Fetal calf serum might fail to induce breaks or at least might protect chromosomes from undergoing breaks to a greater extent than horse serum.

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