A model phage display subtraction method with potential for analysis of differential gene expression
- 5 August 1996
- journal article
- Published by Wiley in FEBS Letters
- Vol. 391 (1-2) , 71-75
- https://doi.org/10.1016/0014-5793(96)00703-x
Abstract
In order to establish a subtractive procedure that makes it possible to enrich selectively phage displayed antibodies directed against proteins constituting a difference between two populations of cells, a competitive selection strategy utilising two solid phases was developed and tested. Antibodies recognising a defined difference between two otherwise identical protein mixtures were isolated and their specificity confirmed. To test further the efficacy of selection inhibition during the competitive selections, selections towards a total cell extract were performed with and without competition from the same extract. An analysis of the resulting phage antibodies confirmed the subtractive nature of the system described.Keywords
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