Discrepancies Between Morphologic, Cytochemical, and Immunologic Characteristics in Acute Myeloblastic Leukemia

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Abstract
This study was designed to compare the cytochemicai pattern with the immunologic phenotype in 108 cases of acute myeloblastic leukemia (AML) classified according to the French–American–British (FAB) criteria. Special attention was paid to the cases where discrepancy existed between these approaches and to a group of 11 patients considered as unclassifiable mainly because a second cell population—megakaryoblastic—was detected. Three types of discrepancies were observed: (1) cases with typical morphologic characteristics and cytochemistry but devoid of lineage-specific antigens; these mainly include poorly differentiated leukemias (eight Ml, four M2, and eight M5a), suggesting that the cytochemicai enzymes are earlier myeloid markers than the currently available monoclonal antibodies; (2) cases in which immunologic characteristics were discordant with morphologic characteristics and cytochemistry; these include two M2 cases positive for monocytic monoclonal antibodies (CD14); six M5b cases positive for granulocytic monoclonal antibodies (CD15); and seven M4 cases lacking in CD14 or CD15 antigens; (3) cases with discrepancies between morphologic characteristics and cytochemistry and in which the immunologic markers permitted the correct assessment of cell lineage (six cases). These results show that the classification of these patients is better achieved by a combined morphologic, cytochemicai, and immunologic approach.

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