A New Strain of Claviceps purpurea Accumulating Tetracyclic Clavine Alkaloids

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Abstract
A new strain of Claviceps was isolated from a blokked mutant of Claviceps purpurea. This strain accumulates substantial amounts of clavine alkaloids (2 g/l). The alkaloid fraction is composed of chanoclavine-I (∼ 10%) and a mixture of agroclavine/elymoclavine (90%). Most suitable for alkaloid production in submerged culture is an ammoncitrate/sucrose medium. The genealogy of the new strain, designated Pepty 695/ch-I is the following one: Pepty 695/S (ergotoxine producer) ⇒ Pepty 695/ch (secoergoline producer) ⇒ Pepty 695/ch-I (tetracyclic clavine producer).

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