Induction of rat duodenal alkaline phosphatase activity by bile in vitro.

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Abstract
The effect of the bile, an inductive factor of duodenal alkaline phosphatase (Alpase) in rats with surgically produced bile duct ligation and in organ cultures, was investigated by biochemical and cytochemical methods. Surgical bile duct ligation induced Alpase activity on the duodenal cells and also rats injected with bile from rats with bile duct ligation showed a great increase in activity. Duodenum from 1-month-old rats could best be maintained for 24 hr in a modified millipore filter roller tube using DM-153 medium without serum. Results in organ culture confirmed the observation in vivo and showed the induction of Alpase activity in the duodenum after administration of the bile and the depression of the rate of Alpase induction by the bile under tunicamycin presence. These results suggest that the bile from rats with bile duct ligation contains an inductive factor of Alpase in the duodenal cells. Organ culture is a very useful method and good model for the analysis of inductive factor of some substances in cell or tissues. Our findings are discussed in this paper.

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