Synthesis of bile acid monosulphates by the isolated perfused rat kidney

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Abstract
Perfusion of an isolated rat kidney with labeled bile acids, in a protein-free medium, resulted in the urinary excretion of the labeled bile acid, 3% being converted into polar metabolites in 1 h. These metabolites were neither glycine nor taurine conjugates, nor bile acid glucuronides, and on solvolysis yielded the free bile acid. Using TLC the metabolite of [24-14C]lithocholic acid had the mobility of lithocholate 3-sulfate. The principal metabolite of [24-14C]chenodeoxycholic acid had the mobility of chenodeoxycholate 7-sulfate; trace amounts appeared as chenodeoxycholate 3-sulfate. [35S]Sulfate was incorporated into chenodeoxycholic acid by the kidney, resulting in a similar pattern of sulfation. No disulfate salt of chenodeoxycholic acid was detected. These findings lend support to the hypothesis that renal synthesis may account for some of the bile acid sulfates present in urine in the cholestatic syndrome in man.