Molecular cloning of the region of the terminus of Escherichia coli K-12 DNA replication
- 1 February 1983
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 153 (2) , 604-609
- https://doi.org/10.1128/jb.153.2.604-609.1983
Abstract
A series of plasmids were isolated by ligation of defined restriction fragments to plasmid pBR325 or by screening of a cosmid bank by in situ colony hybridization. Together with 1 previously isolated plasmid, they spanned 86% of the 30.5- to 34-min region of the genetic map of E. coli K-12. Physical analysis of these plasmids and hybridizations to Southern blots confirmed the endonuclease map of this region, with the exception of a 9.3-kilobase pair inversion.This publication has 25 references indexed in Scilit:
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