RELATION BETWEEN HIGH-ENERGY PHOSPHATE AND LETHAL INJURY IN MYOCARDIAL ISCHEMIA IN DOG

Google Scholar
  • 1 January 1978
    • journal article
    • research article
    • Vol. 92  (1) , 187-+
Abstract
Myocardial ischemia was induced by proximal occlusion of the circumflex coronary artery for 15, 30, 40 or 60 min. Cell injury in the severely ischemic posterior papillary muscle (PP) was evaluated by EM and by measuring the capacity of slices of the injured PP to maintain electrolytes, resynthesize high energy phosphate and exclude inulin during in vitro incubation. ATP content in the ischemic myocardium decreased to 35%, 9%, 7% and 5% of control values after 15, 30, 40 and 60 min of ischemia, respectively, and was associated with a corresponding depletion of total adenine nucleotides. The loss of 65% of the ATP and 15 min of ischemia (reversible injury) was associated with only minimal ultrastructural changes and no significant defects of electrolytes in incubated slices. The depletion of over 90% of the ATP after 40 min of ischemia (irreversible injury) was associated with significant fine structural changes and markedly altered cell volume regulation. A close relationship is suggested between the marked depletion of high energy phosphates and the development of lethal injury in acutely ischemic myocardium.

This publication has 51 references indexed in Scilit: